Nowadays, the recovery of value-added compounds through pretreatment and extraction has become essential for the recycling and conversion of bio-waste. In order to achieve efficient recycling of biowaste and avoid the disadvantages of solvent and time consumption in conventional pretreatment, an environmentally friendly and simple method for the efficient separation and enrichment of luteolin (LTL) from honeysuckle leaves was developed above the green synthesis of novel magnetic, molecularly imprinted polymers (Fe3Ö4th-LTL-MIPs). Fe3Ö4th-LTL-MIPs were made in aqueous solution using polyethyleneimine and dopamine as environmentally friendly bifunctional monomers above Layer by layer on the amino-modified Fe3Ö4th Carrier. The thin sandwich-biscuit-like embossed layer in Fe3Ö4th-LTL-MIPs provide sufficient binding sites with improved uniformity and accessibility for the template molecules, which enables excellent recognition and rapid mass transfer. The nanomaterials obtained show a homogeneous morphology, good crystallinity, satisfactory superparamagnetism, ultrafast adsorption kinetics, high recognition ability and excellent reusability. In addition, Fe3Ö4th-LTL-MIPs as solid phase extraction adsorbents show a high efficiency in the separation and enrichment of LTL from the honeysuckle leaves in combination with HPLC analyzes. Hence the Fe3Ö4th– LTL MIPs could potentially be used as a viable and efficient way to achieve green chemistry goals for selective recycling and the enrichment of target compounds from intricate bio-waste matrices.