The hydroxyl groups in addition to the β-1,4-glycosidic bonds in cellulose provide a crystalline structure, which makes depolymerization a difficult task. In the present study, a green synthesized nanomaterial, functionalized low-layer graphene (FFG), which only has properties that mimic cellulase enzymes, was used for the depolymerization of cellulose units derived from corn cobs (CC). Here we examined the combined effect of cellulase and a very low concentration of FFG (0.02 wt% of biomass) on CC-derived cellulose hydrolysis, which is a 38% increase in hydrolysis compared to the control (i.e. without FFG) showed. Most importantly, hydrolysis experiments were carried out under mild conditions, ie at 50 ° C, producing fermentable sugars without the formation of inhibitor compounds. Furthermore, hydrolyzed CC cellulose was used for the bioethanol fermentation of Saccharomyces cerevisiae, which produced 1.53 times more ethanol in the presence of FFG. The remaining solids content was further used to form cellulose-based nanocomposite films. Here we have tried to develop a zero waste process by using every component that can be an attractive sustainable option. To the best of our knowledge, this is the first report on the claim of cellulase-mimicking activity of FFG nanosheets and its application in the hydrolysis of CC cellulose for improved bioethanol production, which will address the need for expensive cellulase enzymes required for biomass hydrolysis. can make superfluous. Therefore, the FFG-associated biomass-to-bioethanol process can pave the way for the development of a green sustainable biofuel technology for its most important use as a transport fuel.

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